Search This Blog

Saturday, November 22, 2014

The Next Hot Topic in Autism Research? Immune Cells

From SFARI.org
The Simons Foundation Autism Research Initiative

By Virginia Hughes
November 16, 2014

Some cases of autism may result from glitches in immune cells in the blood: This provocative idea stems from a series of unpublished mouse studies presented to a standing-room-only crowd at the 2014 Society for Neuroscience annual meeting in Washington, D.C.

Mice lacking white blood cells known as T cells show abnormal social behaviors, excessive brain connectivity and hints of brain inflammation, according to a slew of new studies from Jonathan Kipnis’ team at the University of Virginia. All of these patterns have also been observed in people with autism.

Peripheral problem: White blood cells
known as T cells circulate in the
bloodstream but do not enter the brain.
The findings are part of a new wave of research bucking the dogma that the immune and nervous systems don’t interact with each other.

“We’re seeing more and more of it and it’s totally exciting,” says Melissa Bauman, assistant professor of psychiatry and behavioral sciences at the University of California, Davis, who was not involved in the new work. “At this point, we’re all going to have to stop being surprised.”

The
link between the brain and the immune system is in fact somewhat intuitive: “When you get sick, you don’t feel well,” says Anthony Filiano, a postdoctoral fellow in Kipnis’ lab who presented the new findings. “But the fact that the immune system can contribute to normal brain function is fairly new. And it’s really neat because the immune system is a lot easier to treat than synapses in the brain.”

Many studies have found evidence of immune problems in people with autism. For example, postmortem brain tissue from individuals with the disorder
contains abnormal microglia, immune cells that reside only in the brain and serve as markers of inflammation. Because this tissue comes from people of all ages, Filiano notes, “what this really suggests is chronic inflammation in these patients.”

Similarly, some children with autism carry
unusual profiles of immune cells in their blood. For example, a small study in 2009 found that, compared with controls, children with autism and their siblings tend to have fewer white blood cells known as CD4+ T cells.

SCID Story

In one of the new experiments, Filiano looked at mice that do not have T cells because of a genetic mutation. Unlike controls, these so-called ‘SCID mice’ do not show a preference for interacting with other mice over objects, he found.

He and his colleagues then injected the SCID mice with thymocytes — progenitor cells from the thymus that turn into T cells — harvested from normal mice. Four weeks after the transplant, SCID mice no longer show the abnormal social behaviors, Filiano says.

These results are particularly intriguing because T cells do not enter the brain. They aggregate around the meninges — layers of tissue that enclose the brain and spinal cord — and release chemicals that relay messages to the brain. Presumably, the loss of these chemical interactions in SCID mice somehow affects their social behaviors.

In another experiment, Filiano’s team induced social deficits in normal mice by giving them a multiple sclerosis drug that prevents T cells from moving into the meninges.


......................................................................
Related Content
......................................................................

“I think it’s pretty surprising, especially thinking about the meninges,” says Lindsay Hayes, a postdoctoral fellow in Akira Sawa’s laboratory at Johns Hopkins University in Baltimore, who was not involved with the work. “I mean, for such a small population of cells [in the meninges] to have a role in such a complex behavior? That’s crazy.”

The researchers theorize that absent or malfunctioning
T cells in the meninges affects microglia in the brain. In support of that idea, they found that microglia in the SCID mice are abnormally shaped, with less branching than normal. This could be a sign of inflammation, though more experiments are needed to say for sure, Filiano says.

To test more directly whether
microglia are involved in social behavior, Kipnis’ team looked at a different kind of mice — an inbred model called BTBR that is known to be asocial. After four weeks of consuming a drug that depletes microglia, the animals’ social behaviors improved. As soon as they stopped taking the drug, the microglia repopulated and the social deficits returned. This suggests that microglia are somehow involved in the animals’ social deficits, although exactly how is as yet unclear.

Finally, the researchers also found a link between T cells and brain connectivity. Using a technique called resting-state functional connectivity, they found that SCID mice are hyperconnected in autism-related regions, including the prefrontal cortex and the insula. This is
similar to connectivity patterns reported in the brains of people with autism, Filiano says.

Pulling all of the data together, the researchers propose that absent or impaired T cells may cause inflammation in the brain and ultimately affect social behaviors.

Others note that there are still many unknowns in the proposed mechanism. “I think this is a T-cell story. Whether T cells are required for the microglial [effects], that’s a different question altogether,” says
Dimitrios Davalos, staff research scientist at the Gladstone Institutes at the University of California, San Francisco.

Kipnis readily agrees. “We have no clue how it works, but it’s there,” he says.

Kipnis says he hopes to find a subset of children with autism who have distinctly abnormal immune profiles of their blood. If T cells turn out to be a robust biomarker, it could have significant treatment implications.

“If it’s really a T-cell effect, then we can restore T cells, we can replace T cells, we can boost T cells, we can vaccinate to get more T cells, we can do lots of things which we definitely cannot do with microglia, astrocytes or neurons,” Kipnis says. “That’s why it’s very exciting.”

No comments:

Post a Comment